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Registro Completo |
Biblioteca(s): |
Embrapa Semiárido. |
Data corrente: |
29/09/2020 |
Data da última atualização: |
29/09/2020 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
BOMFIM, C. S. G.; SILVA, V. B. da; CURSINO, L. H. S.; MATTOS, W. da S.; SANTOS, J. C. S.; SOUZA, L. S. B. de; DANTAS, B. F.; FREITAS, A. D. S. de; FERNANDES JUNIOR, P. I. |
Afiliação: |
Cláudia Silva Gomes Bomfim, Univasf; Valéria Borges da Silva, UFPB; Luiz Henrique Santos Cursino, Bolsista UPE; Wesley da Silva Mattos, Bolsista UPE; Jéssica Caroline Souza Santos, Bolsista UPE; Layane Silva Barbosa de Souza, Bolsista UFRPE; BARBARA FRANCA DANTAS, CPATSA; Ana Dolores Santiago de Freitas, Bolsista UFRPE; PAULO IVAN FERNANDES JUNIOR, CPATSA. |
Título: |
Endophytic bacteria naturally inhabiting commercial maize seeds occupy different niches and are efficient plant growth-promoting agent. |
Ano de publicação: |
2020 |
Fonte/Imprenta: |
Symbiosis, v. 81, p. 255?269, august 2020. |
ISSN: |
0334-5114 |
DOI: |
https://doi.org/10.1007/s13199-020-00701-z |
Idioma: |
Inglês |
Conteúdo: |
The isolation of seed-endophytic bacteria (SEB) is a promising approach for the selection of maize plant growth-promoting bacteria (PGPB). With the hypothesis that maize seeds harbor SEB that occupy different niches and show plant-growth-promoting abilities, we aimed to isolate and characterize the potential PGPB from these seeds. The bacteria from commercial seeds (BRS Gorutuba) and axenically grown maize-seedlings were isolated, molecularly fingerprinted, and genetically characterized by amplified ribosomal DNA restriction analysis (ARDRA). All SEB were evaluated for their promotion of early root growth. The selected strains were identified by 16S rRNA sequencing and evaluated for their plant growth-promotion traits. A pot experiment was conducted to assess the ability of the SEB to promote maize-growth and nutrient accumulation. Fifty-one bacterial strains were retrieved, mostly isolated directly from the seeds. All the isolated bacteria represented different strains according to their molecular fingerprinting. ARDRA clustering revealed six clusters influenced by their plant tissue/organ of origin. Twenty-nine SEB were selected based on their influence on early root growth. The 16S rRNA sequences classified the SEB as Bacillus (22), Paenibacillus (2) and Acinetobacter (5). The inoculation of Bacillus ESA 674 improved the shoot dry mass in 57% and the Acinetobacter ESA 662 improved the root growth by 235%, both compared to the uninoculated control. At least 12 bacteria improved nutrient content in the shoots. The Bacillus spp. ESA 674 and ESA 652 outstood in improving maize nutrition by increasing the accumulation of several nutrients. MenosThe isolation of seed-endophytic bacteria (SEB) is a promising approach for the selection of maize plant growth-promoting bacteria (PGPB). With the hypothesis that maize seeds harbor SEB that occupy different niches and show plant-growth-promoting abilities, we aimed to isolate and characterize the potential PGPB from these seeds. The bacteria from commercial seeds (BRS Gorutuba) and axenically grown maize-seedlings were isolated, molecularly fingerprinted, and genetically characterized by amplified ribosomal DNA restriction analysis (ARDRA). All SEB were evaluated for their promotion of early root growth. The selected strains were identified by 16S rRNA sequencing and evaluated for their plant growth-promotion traits. A pot experiment was conducted to assess the ability of the SEB to promote maize-growth and nutrient accumulation. Fifty-one bacterial strains were retrieved, mostly isolated directly from the seeds. All the isolated bacteria represented different strains according to their molecular fingerprinting. ARDRA clustering revealed six clusters influenced by their plant tissue/organ of origin. Twenty-nine SEB were selected based on their influence on early root growth. The 16S rRNA sequences classified the SEB as Bacillus (22), Paenibacillus (2) and Acinetobacter (5). The inoculation of Bacillus ESA 674 improved the shoot dry mass in 57% and the Acinetobacter ESA 662 improved the root growth by 235%, both compared to the uninoculated control. At least 12 bacteria imp... Mostrar Tudo |
Palavras-Chave: |
Isolamento de bactérias endofíticas; Nutrição de planta. |
Thesagro: |
Absorção de Nutrientes; Bacilo; Bactéria; Milho; Semente. |
Thesaurus Nal: |
Acinetobacter; Plant growth control; Seed-borne bacteria. |
Categoria do assunto: |
F Plantas e Produtos de Origem Vegetal |
Marc: |
LEADER 02786naa a2200361 a 4500 001 2125163 005 2020-09-29 008 2020 bl uuuu u00u1 u #d 022 $a0334-5114 024 7 $ahttps://doi.org/10.1007/s13199-020-00701-z$2DOI 100 1 $aBOMFIM, C. S. G. 245 $aEndophytic bacteria naturally inhabiting commercial maize seeds occupy different niches and are efficient plant growth-promoting agent.$h[electronic resource] 260 $c2020 520 $aThe isolation of seed-endophytic bacteria (SEB) is a promising approach for the selection of maize plant growth-promoting bacteria (PGPB). With the hypothesis that maize seeds harbor SEB that occupy different niches and show plant-growth-promoting abilities, we aimed to isolate and characterize the potential PGPB from these seeds. The bacteria from commercial seeds (BRS Gorutuba) and axenically grown maize-seedlings were isolated, molecularly fingerprinted, and genetically characterized by amplified ribosomal DNA restriction analysis (ARDRA). All SEB were evaluated for their promotion of early root growth. The selected strains were identified by 16S rRNA sequencing and evaluated for their plant growth-promotion traits. A pot experiment was conducted to assess the ability of the SEB to promote maize-growth and nutrient accumulation. Fifty-one bacterial strains were retrieved, mostly isolated directly from the seeds. All the isolated bacteria represented different strains according to their molecular fingerprinting. ARDRA clustering revealed six clusters influenced by their plant tissue/organ of origin. Twenty-nine SEB were selected based on their influence on early root growth. The 16S rRNA sequences classified the SEB as Bacillus (22), Paenibacillus (2) and Acinetobacter (5). The inoculation of Bacillus ESA 674 improved the shoot dry mass in 57% and the Acinetobacter ESA 662 improved the root growth by 235%, both compared to the uninoculated control. At least 12 bacteria improved nutrient content in the shoots. The Bacillus spp. ESA 674 and ESA 652 outstood in improving maize nutrition by increasing the accumulation of several nutrients. 650 $aAcinetobacter 650 $aPlant growth control 650 $aSeed-borne bacteria 650 $aAbsorção de Nutrientes 650 $aBacilo 650 $aBactéria 650 $aMilho 650 $aSemente 653 $aIsolamento de bactérias endofíticas 653 $aNutrição de planta 700 1 $aSILVA, V. B. da 700 1 $aCURSINO, L. H. S. 700 1 $aMATTOS, W. da S. 700 1 $aSANTOS, J. C. S. 700 1 $aSOUZA, L. S. B. de 700 1 $aDANTAS, B. F. 700 1 $aFREITAS, A. D. S. de 700 1 $aFERNANDES JUNIOR, P. I. 773 $tSymbiosis$gv. 81, p. 255?269, august 2020.
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Registros recuperados : 54 | |
1. | | VIEIRA, L. de J. Conservação in vitro e criopreservação de espécies de Manihot. Feira de Santana: Universidade Estadual de Feira de Santana, 2013. Orientador: Prof. Dr. José Raniere Ferreira de Santana; Co-orientadores: Dra. Fernanda Vidigal Duarte Souza - CNPMF; Dr. Alfredo Augusto Cunha Alves - SRI.Tipo: Orientação de Tese de Pós-Graduação |
Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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7. | | VIEIRA, L. de J.; SOUZA, F. V. D.; ALVES, A. A. C.; VIDAL, M. das G. Produção, tamanho e viabilidade de pólen de variedades domésticas e espécies silvestres de mandioca. In: SEMINÁRIO DE PESQUISA DO RECÔNCAVO DA BAHIA, 3.; SEMINÁRIO ESTUDANTIL DE PESQUISA DA UFRB, 3.; SEMINÁRIO DE PÓS-GRADUAÇÃO DA UFRB, 3., 2009, Cruz das Almas. Evolução do conhecimento e o desenvolvimento social: [resumos...]. Cruz das Almas: Universidade Federal do Recôncavo da Bahia, 2009. 01 CD-ROM.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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16. | | SOARES, T. L.; SOUZA, F. V. D.; SOUZA, E. H. de; VIEIRA, L. de J. Viabilidade de pólen de abacaxizeiro Ananas comosus var. comosus. In: SIMPÓSIO BRASILEIRO DE RECURSOS GENÉTICOS, 2., 2008, Brasília, DF. Anais... Brasília, DF: Embrapa Recursos Genéticos e Biotecnologia, 2008. p. 400.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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18. | | VIEIRA, L. de J.; SOUZA, F. V. D.; ALVES, A. A. C. Viabilidade de pólen de mandioca silvestre sob diferentes condições de armazenamento. In: CONGRESSO BRASILEIRO DE RECURSOS GENÉTICOS; WORKSHOP EM BIOPROSPECÇÃO E CONSERVAÇÃO DE PLANTAS NATIVAS DO SEMI-ÁRIDO, 3.; WORKSHOP INTERNACIONAL SOBRE BIOENERGIA E MEIO AMBIENTE, 2010, Salvador. Bancos de germoplasma: descobrir a riqueza, garantir o futuro: anais. Brasília, DF: Embrapa Recursos Genéticos e Biotecnologia, 2010. 1 CD-ROM (Embrapa Recursos Genéticos e Biotecnologia. Documentos, 304). pdf 485.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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Registros recuperados : 54 | |
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